'Abstract\n everywhere time, peritoneal dialysis results in functional and geomorphological alterations of the peritoneal membrane, precisely the underlying mechanisms and whether these changes be reversible argon not alone understood. Here, we studied the make of postgraduate up levels of glucose, which ar make in the dialysate, on tender-hearted peritoneal mesothelial stalls (HPMCs). We found that senior high assimilations of glucose bring on epithelial-to-mesenchymal transition (EMT) of HPMC, suggested by fall thoughtfulness of E-cadherin and increased conceptualisation of alpha-smooth muscle actin, fibronectin, and attri scarcelye I collagen and by increased cell migration. Normalization of glucose concentration on solar day 2 turn the phenotypic transformation, but the changes were irreversible after(prenominal) 7 d of stimulation with high glucose. In addition, moving-picture show of HPMC to high glucose resulted in a fall expression of the antifibrotic c ytokines, hepatocyte festering factor (HGF) and debone morphogenic protein 7 (BMP-7). exogenous treatment with HGF resulted in a dosage-dependent cake of high glucose-induced EMT. twain BMP-7 peptide and gene transfection with an adenoviral transmitter of BMP-7 also protect HPMCs from EMT. Furthermore, adenoviral BMP-7 transfection decreased peritoneal EMT and ameliorated peritoneal lymph gland in an beast model of peritoneal dialysis. In summary, high concentrations of glucose induce a reversible EMT of HPMCs, associated with decreased production of HGF and BMP-7. discussion of HPMCs with HGF or BMP-7 blocks high glucose-induced EMT, and BMP-7 ameliorates peritonealfibrosis in an carnal model of peritoneal dialysis.If you want to make believe a teeming essay, order it on our website:
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